Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis
Само за регистроване кориснике
1979
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and B. parapertussis has been investigated. Cultures were grown on Bordet-Gengou medium, mixed with fresh guinea-pig serum and injected into mice by the intraperitoneal route. Much lower LDse values were found when the animals were injected with mixtures of B. pertussis and guinea-pig serum. The cultures of 12 B. pertussis strains showed LDses between 1·10 and 3·25 billion bacteria after the addition of fresh guinea-pig serum. The LDse values of the control cultures ranged between 2·68 and 8·06 billion bacteria. Compared with the controls the LDses of the cultures mixed with fresh guinea-pig serum were significantly lower (P<0·05). Increased mortality was observed when cultures of B. pertussis of low toxicity were mixed with fresh guinea-pig serum and injected into mice. Such cultures after the addition of serum caused approximately 100% mortality in the injected animals. The effect of fresh guinea-pig seru...m on the toxicity of B. parapertussis cultures was assayed. Cultures highly toxic for mice were less toxic after the addition of fresh guinea-pig serum. The cultures of toxic strains which caused 50% mortality failed to kill mice after addition of fresh guinea-pig serum. Cultures of B. pertussis and B. parapertussis strains tested for toxicity showed variable susceptibility to the bactericidal action of guinea-pig serum. In cultures of both Bordetella species between 60 and 99% of the bacteria were killed by serum. No correlation between toxicity and the bactericidal activity of fresh guinea-pig serum toward B. pertussis and B. parapertussis strains was observed.
Извор:
Journal of Biological Standardization, 1979, 7, 4, 285-292
DOI: 10.1016/S0092-1157(79)80002-5
ISSN: 0092-1157
PubMed: 231604
Scopus: 2-s2.0-0018612778
Институција/група
TorlakTY - JOUR AU - Spasojević-Rabrenović, V. PY - 1979 UR - http://intor.torlakinstitut.com/handle/123456789/19 AB - The effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and B. parapertussis has been investigated. Cultures were grown on Bordet-Gengou medium, mixed with fresh guinea-pig serum and injected into mice by the intraperitoneal route. Much lower LDse values were found when the animals were injected with mixtures of B. pertussis and guinea-pig serum. The cultures of 12 B. pertussis strains showed LDses between 1·10 and 3·25 billion bacteria after the addition of fresh guinea-pig serum. The LDse values of the control cultures ranged between 2·68 and 8·06 billion bacteria. Compared with the controls the LDses of the cultures mixed with fresh guinea-pig serum were significantly lower (P<0·05). Increased mortality was observed when cultures of B. pertussis of low toxicity were mixed with fresh guinea-pig serum and injected into mice. Such cultures after the addition of serum caused approximately 100% mortality in the injected animals. The effect of fresh guinea-pig serum on the toxicity of B. parapertussis cultures was assayed. Cultures highly toxic for mice were less toxic after the addition of fresh guinea-pig serum. The cultures of toxic strains which caused 50% mortality failed to kill mice after addition of fresh guinea-pig serum. Cultures of B. pertussis and B. parapertussis strains tested for toxicity showed variable susceptibility to the bactericidal action of guinea-pig serum. In cultures of both Bordetella species between 60 and 99% of the bacteria were killed by serum. No correlation between toxicity and the bactericidal activity of fresh guinea-pig serum toward B. pertussis and B. parapertussis strains was observed. T2 - Journal of Biological Standardization T1 - Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis EP - 292 IS - 4 SP - 285 VL - 7 DO - 10.1016/S0092-1157(79)80002-5 ER -
@article{ author = "Spasojević-Rabrenović, V.", year = "1979", abstract = "The effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and B. parapertussis has been investigated. Cultures were grown on Bordet-Gengou medium, mixed with fresh guinea-pig serum and injected into mice by the intraperitoneal route. Much lower LDse values were found when the animals were injected with mixtures of B. pertussis and guinea-pig serum. The cultures of 12 B. pertussis strains showed LDses between 1·10 and 3·25 billion bacteria after the addition of fresh guinea-pig serum. The LDse values of the control cultures ranged between 2·68 and 8·06 billion bacteria. Compared with the controls the LDses of the cultures mixed with fresh guinea-pig serum were significantly lower (P<0·05). Increased mortality was observed when cultures of B. pertussis of low toxicity were mixed with fresh guinea-pig serum and injected into mice. Such cultures after the addition of serum caused approximately 100% mortality in the injected animals. The effect of fresh guinea-pig serum on the toxicity of B. parapertussis cultures was assayed. Cultures highly toxic for mice were less toxic after the addition of fresh guinea-pig serum. The cultures of toxic strains which caused 50% mortality failed to kill mice after addition of fresh guinea-pig serum. Cultures of B. pertussis and B. parapertussis strains tested for toxicity showed variable susceptibility to the bactericidal action of guinea-pig serum. In cultures of both Bordetella species between 60 and 99% of the bacteria were killed by serum. No correlation between toxicity and the bactericidal activity of fresh guinea-pig serum toward B. pertussis and B. parapertussis strains was observed.", journal = "Journal of Biological Standardization", title = "Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis", pages = "292-285", number = "4", volume = "7", doi = "10.1016/S0092-1157(79)80002-5" }
Spasojević-Rabrenović, V.. (1979). Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis. in Journal of Biological Standardization, 7(4), 285-292. https://doi.org/10.1016/S0092-1157(79)80002-5
Spasojević-Rabrenović V. Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis. in Journal of Biological Standardization. 1979;7(4):285-292. doi:10.1016/S0092-1157(79)80002-5 .
Spasojević-Rabrenović, V., "Effect of fresh guinea-pig serum on the toxicity of Bordetella pertussis and Bordetella parapertussis" in Journal of Biological Standardization, 7, no. 4 (1979):285-292, https://doi.org/10.1016/S0092-1157(79)80002-5 . .