Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages

Abstract

In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation.

U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije.