Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages
Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga
Autori
Živančević-Simonović, SnežanaInić-Kanada, Aleksandra
Stojanović, Marijana
Dimitrijević, Ljiljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number o...f macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation.
U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovel...o je do smanjenja T limfocitne proliferacije.
Ključne reči:
macrophage / antigen-presenting cells / sheep redblood cells (SRBC) / mixed leukocyte reaction (MLR) / mouseIzvor:
Acta veterinaria - Beograd, 2004, 54, 5-6, 327-335Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0406327Z
ISSN: 0567-8315
WoS: 000226086100001
Scopus: 2-s2.0-13244297001
Institucija/grupa
TorlakTY - JOUR AU - Živančević-Simonović, Snežana AU - Inić-Kanada, Aleksandra AU - Stojanović, Marijana AU - Dimitrijević, Ljiljana PY - 2004 UR - http://intor.torlakinstitut.com/handle/123456789/187 AB - In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation. AB - U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta veterinaria - Beograd T1 - Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages T1 - Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga EP - 335 IS - 5-6 SP - 327 VL - 54 DO - 10.2298/AVB0406327Z ER -
@article{ author = "Živančević-Simonović, Snežana and Inić-Kanada, Aleksandra and Stojanović, Marijana and Dimitrijević, Ljiljana", year = "2004", abstract = "In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation., U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta veterinaria - Beograd", title = "Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages, Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga", pages = "335-327", number = "5-6", volume = "54", doi = "10.2298/AVB0406327Z" }
Živančević-Simonović, S., Inić-Kanada, A., Stojanović, M.,& Dimitrijević, L.. (2004). Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages. in Acta veterinaria - Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(5-6), 327-335. https://doi.org/10.2298/AVB0406327Z
Živančević-Simonović S, Inić-Kanada A, Stojanović M, Dimitrijević L. Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages. in Acta veterinaria - Beograd. 2004;54(5-6):327-335. doi:10.2298/AVB0406327Z .
Živančević-Simonović, Snežana, Inić-Kanada, Aleksandra, Stojanović, Marijana, Dimitrijević, Ljiljana, "Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages" in Acta veterinaria - Beograd, 54, no. 5-6 (2004):327-335, https://doi.org/10.2298/AVB0406327Z . .