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dc.creatorŠeatović, Saša S.
dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.creatorŽivković, Irena
dc.creatorJankov, Ratko
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2021-02-18T10:27:06Z
dc.date.available2021-02-18T10:27:06Z
dc.date.issued2004
dc.identifier.issn1532-1819
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/181
dc.description.abstractAccording to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily extrapolated to the estimation of vaccine potency.en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.rightsrestrictedAccess
dc.sourceJournal of Immunoassay & Immunochemistry
dc.subjectsandwich ELISAen
dc.subjecttetanus toxoiden
dc.subjectmonoclonal antibodiesen
dc.titleDevelopment of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentrationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage44
dc.citation.issue1
dc.citation.other25(1): 31-44
dc.citation.rankM23
dc.citation.spage31
dc.citation.volume25
dc.identifier.doi10.1081/IAS-120027224
dc.identifier.pmid15038615
dc.identifier.rcubconv_148
dc.identifier.scopus2-s2.0-18844466254
dc.identifier.wos000220152400003
dc.type.versionpublishedVersion


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