The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors
Uticaj opioidnih peptida na produkciju superoksid anjona u peritonealnim makrofagama pacova stimulisanim zimozanom - uloga µ, δ i κ opioidnih receptora
dc.creator | Vujić, Vesna | |
dc.creator | Stanojević, Stanislava | |
dc.creator | Dimitrijević, Mirjana | |
dc.date.accessioned | 2021-02-18T10:23:59Z | |
dc.date.available | 2021-02-18T10:23:59Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/137 | |
dc.description.abstract | It is well documented that endogenous opioid peptides modulate the activity of immune and inflammatory cells. Opioid peptides exert biological effects through at least three opioid receptor types, known as µ, δ and κ. In the present study we have investigated which types of opioid receptors are involved in the opioid peptide-induced modulation of superoxide an/on release from macrophages. Rat peritoneal macrophages were stimulated with zymosan, and treated in vitro with opioid peptides and/or antagonists specific for different opioid receptor types. The results showed that the µ opioid receptor agonist, beta-endorphin, and the k opioid receptor agonist dynorphin A, decreased superoxide anion production. Conversely methionine-enkephalin (Met-Enk) and leucine-enkephalin, that have a preference for δ opioid receptors, did not affect superoxide anion release. Furthermore, we have tested the effect of Met-Enk on superoxide anion release from macrophages under blockade of all receptors with specific antagonists except for one receptor subtype. Accordingly, it was found that the Met-Enk induced increase and decrease of superoxide anion production were mediated through δ1,2 and µκ opioid receptors, respectively. It can be concluded that opposing activity of µ, and κ receptors vs. δ receptors together with the affinity for a ceflain receptor-type determines the effect of a particular endogenous opioid peptide on superoxide anion release from rat peritoneal macrophages. | en |
dc.description.abstract | Poznato je da endogeni opioidni peptidi modulišu aktivnost imunskih i inflamatornih ćelija. Opioidni peptidi ostvaruju biološke efekte preko tri tipa receptora, µ, δ i κ. U ovom radu ispitivali smo koji tipovi opioidnih receptora učestvuju u opioidnoj modulaciji oslobadanjasuperoksid anjona iz peritonealnih makrofaga. Peritonealne makrofage pacova su stimulisane zimozanom, i tretirane in vitro sa opioidnim peptidima i/ili specifičnim antagonistima različitih tipova opioidnih receptora. Rezultati su pokazali da beta-endorphin, agonist µ, opiodnih receptora, i dynorphin A, agonist κ opiodnih receptora, smanjuju produkciju superoksid anjona. S druge strane metionin-enkefalin (Met-Enk) i leucin-enkefalin, koji se prevashodno vezuju za δ opioidne receptore, nisu uticali na oslobadanje superoksid anjona. Pored toga, ispitivali smo uticaj Met-Enk na oslobađanje superoksid anjona iz makrofaga u uslovima blokade svih receptora sa specifičnim antagonistima izuzev jednog tipa/podtipa opioidnog receptora. Shodno tome pokazali smo da Met-Enk povećava oslobadanje superoksid anjona preko δ1,2 a smanjuje oslobadanje superoksid anjona preko µk opioidnih receptora. Može se zaključiti da je uticaj endogenih opioidnih peptida na oslobadanje superoksid anjona iz peritonealnih makrofaga pacova određen suprotnom aktivnošću (µ i k receptora u odnosu na δ receptore, kao i različitim afinitetom peptida za pojedine tipove opioidnih receptora. | sr |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.rights | openAccess | |
dc.source | Acta veterinaria - Beograd | |
dc.subject | macrophages | en |
dc.subject | methionine-enkephalin | en |
dc.subject | opioid receptors µ | en |
dc.subject | δ and κ | en |
dc.subject | superoxide anion | en |
dc.title | The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors | en |
dc.title | Uticaj opioidnih peptida na produkciju superoksid anjona u peritonealnim makrofagama pacova stimulisanim zimozanom - uloga µ, δ i κ opioidnih receptora | sr |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 78 | |
dc.citation.issue | 2-3 | |
dc.citation.other | 52(2-3): 69-78 | |
dc.citation.rank | M23 | |
dc.citation.spage | 69 | |
dc.citation.volume | 52 | |
dc.identifier.doi | 10.2298/AVB0203069V | |
dc.identifier.fulltext | http://intor.torlakinstitut.com/bitstream/id/28/134.pdf | |
dc.identifier.scopus | 2-s2.0-0036412747 | |
dc.identifier.wos | 000176114300001 | |
dc.type.version | publishedVersion |