Maturation of IgG avidity to individual rubella virus structural proteins
Samo za registrovane korisnike
2001
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturati...on. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.
Ključne reči:
expression / rubella virus / structural proteins / baculovirus / avidityIzvor:
Journal of Clinical Virology, 2001, 22, 1, 47-54Izdavač:
- Elsevier, Amsterdam
DOI: 10.1016/S1386-6532(01)00161-5
ISSN: 1386-6532
PubMed: 11418352
WoS: 000169985600005
Scopus: 2-s2.0-0034966909
Institucija/grupa
TorlakTY - JOUR AU - Nedeljković, Jasminka AU - Jovanović, T. AU - Oker-Blom, C. PY - 2001 UR - http://intor.torlakinstitut.com/handle/123456789/127 AB - Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - Journal of Clinical Virology T1 - Maturation of IgG avidity to individual rubella virus structural proteins EP - 54 IS - 1 SP - 47 VL - 22 DO - 10.1016/S1386-6532(01)00161-5 ER -
@article{ author = "Nedeljković, Jasminka and Jovanović, T. and Oker-Blom, C.", year = "2001", abstract = "Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Journal of Clinical Virology", title = "Maturation of IgG avidity to individual rubella virus structural proteins", pages = "54-47", number = "1", volume = "22", doi = "10.1016/S1386-6532(01)00161-5" }
Nedeljković, J., Jovanović, T.,& Oker-Blom, C.. (2001). Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology Elsevier, Amsterdam., 22(1), 47-54. https://doi.org/10.1016/S1386-6532(01)00161-5
Nedeljković J, Jovanović T, Oker-Blom C. Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology. 2001;22(1):47-54. doi:10.1016/S1386-6532(01)00161-5 .
Nedeljković, Jasminka, Jovanović, T., Oker-Blom, C., "Maturation of IgG avidity to individual rubella virus structural proteins" in Journal of Clinical Virology, 22, no. 1 (2001):47-54, https://doi.org/10.1016/S1386-6532(01)00161-5 . .