InTOR - Repository of the Institute “Torlak”
Institute of Virology, Vaccines and Sera “Torlak”
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Maturation of IgG avidity to individual rubella virus structural proteins

Authorized Users Only
2001
Authors
Nedeljković, Jasminka
Jovanović, T.
Oker-Blom, C.
Article (Published version)
Metadata
Show full item record
Abstract
Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturati...on. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.

Keywords:
expression / rubella virus / structural proteins / baculovirus / avidity
Source:
Journal of Clinical Virology, 2001, 22, 1, 47-54
Publisher:
  • Elsevier, Amsterdam

DOI: 10.1016/S1386-6532(01)00161-5

ISSN: 1386-6532

PubMed: 11418352

WoS: 000169985600005

Scopus: 2-s2.0-0034966909
[ Google Scholar ]
19
17
URI
http://intor.torlakinstitut.com/handle/123456789/127
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Nedeljković, Jasminka
AU  - Jovanović, T.
AU  - Oker-Blom, C.
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/127
AB  - Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Clinical Virology
T1  - Maturation of IgG avidity to individual rubella virus structural proteins
EP  - 54
IS  - 1
SP  - 47
VL  - 22
DO  - 10.1016/S1386-6532(01)00161-5
UR  - conv_124
ER  - 
@article{
author = "Nedeljković, Jasminka and Jovanović, T. and Oker-Blom, C.",
year = "2001",
abstract = "Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Clinical Virology",
title = "Maturation of IgG avidity to individual rubella virus structural proteins",
pages = "54-47",
number = "1",
volume = "22",
doi = "10.1016/S1386-6532(01)00161-5",
url = "conv_124"
}
Nedeljković, J., Jovanović, T.,& Oker-Blom, C.. (2001). Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology
Elsevier, Amsterdam., 22(1), 47-54.
https://doi.org/10.1016/S1386-6532(01)00161-5
conv_124
Nedeljković J, Jovanović T, Oker-Blom C. Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology. 2001;22(1):47-54.
doi:10.1016/S1386-6532(01)00161-5
conv_124 .
Nedeljković, Jasminka, Jovanović, T., Oker-Blom, C., "Maturation of IgG avidity to individual rubella virus structural proteins" in Journal of Clinical Virology, 22, no. 1 (2001):47-54,
https://doi.org/10.1016/S1386-6532(01)00161-5 .,
conv_124 .

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB