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dc.creatorVujić-Redžić, V.
dc.creatorDimitrijević, Mirjana
dc.creatorStanojević, Stanislava
dc.creatorKovačević-Jovanović, Vesna
dc.creatorMiletić, Tatjana
dc.creatorRadulović, Jelena
dc.date.accessioned2021-02-18T10:22:34Z
dc.date.available2021-02-18T10:22:34Z
dc.date.issued2000
dc.identifier.issn1021-7401
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/115
dc.description.abstractMethionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.en
dc.publisherKarger, Basel
dc.rightsrestrictedAccess
dc.sourceNeuroimmunomodulation
dc.subjectmethionine-enkephalinen
dc.subjectanti-methionine-enkephalinen
dc.subjectantibodiesen
dc.subjectpaw edemaen
dc.subjectperitoneal macrophagesen
dc.subjectH2O2 releaseen
dc.subjectbehavioren
dc.subjectraten
dc.titlePeripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the raten
dc.typearticle
dc.rights.licenseARR
dc.citation.epage77
dc.citation.issue2
dc.citation.other8(2): 70-77
dc.citation.rankM21
dc.citation.spage70
dc.citation.volume8
dc.identifier.doi10.1159/000026455
dc.identifier.pmid10965231
dc.identifier.rcubconv_117
dc.identifier.scopus2-s2.0-0033803297
dc.identifier.wos000089014700003
dc.type.versionpublishedVersion


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