Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients
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1999
Authors
Uzelac-Keserović, B.Spasić, P.
Bojanić, N.
Dimitrijević, J.
Lako, Branislav
Lepšanović, Z.
Kuljić-Kapulica, N.
Vasić, D.
Apostolov, K.
Article (Published version)
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Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using ...the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.
Keywords:
Endemic balkan nephropathy / Isolation of coronavirusSource:
Nephron, 1999, 81, 2, 141-145Publisher:
- S. Karger AG
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Institution/Community
TorlakTY - JOUR AU - Uzelac-Keserović, B. AU - Spasić, P. AU - Bojanić, N. AU - Dimitrijević, J. AU - Lako, Branislav AU - Lepšanović, Z. AU - Kuljić-Kapulica, N. AU - Vasić, D. AU - Apostolov, K. PY - 1999 UR - http://intor.torlakinstitut.com/handle/123456789/109 AB - Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis. PB - S. Karger AG T2 - Nephron T1 - Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients EP - 145 IS - 2 SP - 141 VL - 81 DO - 10.1159/000045269 ER -
@article{ author = "Uzelac-Keserović, B. and Spasić, P. and Bojanić, N. and Dimitrijević, J. and Lako, Branislav and Lepšanović, Z. and Kuljić-Kapulica, N. and Vasić, D. and Apostolov, K.", year = "1999", abstract = "Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.", publisher = "S. Karger AG", journal = "Nephron", title = "Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients", pages = "145-141", number = "2", volume = "81", doi = "10.1159/000045269" }
Uzelac-Keserović, B., Spasić, P., Bojanić, N., Dimitrijević, J., Lako, B., Lepšanović, Z., Kuljić-Kapulica, N., Vasić, D.,& Apostolov, K.. (1999). Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients. in Nephron S. Karger AG., 81(2), 141-145. https://doi.org/10.1159/000045269
Uzelac-Keserović B, Spasić P, Bojanić N, Dimitrijević J, Lako B, Lepšanović Z, Kuljić-Kapulica N, Vasić D, Apostolov K. Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients. in Nephron. 1999;81(2):141-145. doi:10.1159/000045269 .
Uzelac-Keserović, B., Spasić, P., Bojanić, N., Dimitrijević, J., Lako, Branislav, Lepšanović, Z., Kuljić-Kapulica, N., Vasić, D., Apostolov, K., "Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients" in Nephron, 81, no. 2 (1999):141-145, https://doi.org/10.1159/000045269 . .