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Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins

Authorized Users Only
1999
Authors
Ćirković, Tanja
Bukilica, Mirjana
Gavrović, Marija
Vujčić, Zoran
Petrović, S.
Jankov, Ratko
Article (Published version)
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Abstract
Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic va...lues. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.

Keywords:
allergoid / chemical modification / Dactylis glomerata / maleic anhydride / orchard grass / skin prick test / succinic anhydride
Source:
Allergy, 1999, 54, 2, 128-134
Publisher:
  • Wiley, Hoboken

DOI: 10.1034/j.1398-9995.1999.00778.x

ISSN: 0105-4538

PubMed: 10221435

WoS: 000079413400005

Scopus: 2-s2.0-0000670417
[ Google Scholar ]
22
20
URI
http://intor.torlakinstitut.com/handle/123456789/104
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Ćirković, Tanja
AU  - Bukilica, Mirjana
AU  - Gavrović, Marija
AU  - Vujčić, Zoran
AU  - Petrović, S.
AU  - Jankov, Ratko
PY  - 1999
UR  - http://intor.torlakinstitut.com/handle/123456789/104
AB  - Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.
PB  - Wiley, Hoboken
T2  - Allergy
T1  - Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins
EP  - 134
IS  - 2
SP  - 128
VL  - 54
DO  - 10.1034/j.1398-9995.1999.00778.x
UR  - conv_94
ER  - 
@article{
author = "Ćirković, Tanja and Bukilica, Mirjana and Gavrović, Marija and Vujčić, Zoran and Petrović, S. and Jankov, Ratko",
year = "1999",
abstract = "Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins",
pages = "134-128",
number = "2",
volume = "54",
doi = "10.1034/j.1398-9995.1999.00778.x",
url = "conv_94"
}
Ćirković, T., Bukilica, M., Gavrović, M., Vujčić, Z., Petrović, S.,& Jankov, R.. (1999). Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy
Wiley, Hoboken., 54(2), 128-134.
https://doi.org/10.1034/j.1398-9995.1999.00778.x
conv_94
Ćirković T, Bukilica M, Gavrović M, Vujčić Z, Petrović S, Jankov R. Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy. 1999;54(2):128-134.
doi:10.1034/j.1398-9995.1999.00778.x
conv_94 .
Ćirković, Tanja, Bukilica, Mirjana, Gavrović, Marija, Vujčić, Zoran, Petrović, S., Jankov, Ratko, "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins" in Allergy, 54, no. 2 (1999):128-134,
https://doi.org/10.1034/j.1398-9995.1999.00778.x .,
conv_94 .

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