@misc{
author = "Kosanović, Dejana and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Figure S1. Produce Phase III substrate which was heavily colonised by T. aggressivum and used as inoculum for subsequent experiment; Figure S2. Most probable number (MPN) analysis results, a) -2 dilution level, b) -5 dilution level; Figure S3. PCR verification of T. aggressivum f. europaeum. Wells 2,3,4 are negativeve control (culture FM5 T. harzianum). Wells 5,6,7 are T. aggressivum FM10. Wells 6,7 are very faint in the image unfortunately, but they were on the gel. We can still slightly see them. Sample CBS 100526 is in wells 8,9,10 and they are all clearly positive. Well 8 is Trichoderma universal (ITS1/4 primers), well 9 is for both Th2/Th4 biotypes (TH1 INT/ITS4 primers). Th2 biotype is T. aggressivum f. europaeum, Th4 biotype is T. aggressivum f. aggressivum, and in well 10 is Th2 biotype specific (i.e. T. aggressivum f. europium, 18S/TH1 INT REV primers); Figure S4. Principal component analysis (PCA statistical procedure). ▫ - T. aggressivum group and ▫ - Control group; Figure S5 a. Increase/Decrease in biological processes in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 b. Increase/Decrease in molecular function in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 c. Increased/decreased cellular components in A. bisporus after 4 day treatment with T. aggressivum 48h supernatant; Figure S5 d. Increase/decrease in enzyme activity of A. bisporus after 4 day of treatment with 48h T. aggressivum supernatant; Figure S6. Green mold was observed on the mushroom casing of inoculated plots (c:10-3 and b:10-4), but not on the control plots (a). Starting from day 14th dense white mycelia was observed, after few days the color changed into green after extensive sporulation (b, c); Figure S7. Symptoms of green mould (deformities of sporocarp and brown spots) on fruiting body on day 17th after casing the compost; Table S1. Colorimetry assay on mushroom pilei in infected or controlled plots; Figure S8. Proteomic responses of A. bisporus following 25-day incubation with 10-4 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S9. Proteomic responses of A. bisporus following 25-day incubation with -3 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S10 a. Increased molecular function 25 days after A. bisporus inoculation with T. aggressivum; Figure S10 b. Increased cellular component day 24 after A. bisporus inoculation with T. aggressivum; Figure S10 c. Increased enzymes day 25 after inoculation of A. bisporus with T. aggressivum; Figure S11a. Proteomic responses of A. bisporus following 2 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S11b. Proteomic responses of A. bisporus following 8 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed",
journal = "Fungal Biology",
title = "Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003.",
pages = "820-814",
number = "9",
volume = "124",
url = "https://hdl.handle.net/21.15107/rcub_intor_640"
}
